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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
30/05/2019 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
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Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
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Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
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DOI : |
10.1002/pca.2837 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
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Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
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Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
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Asunto categoría : |
K11 Ingeniería forestal |
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Marc : |
LEADER 02910naa a2200241 a 4500
001 1059792
005 2020-05-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/pca.2837$2DOI
100 1 $aWALLACE, F.
245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.
520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.
653 $aIMMUNOADJUVANT SAPONINS
653 $aMASS SPECTROMETRY
653 $aMETHYLATION ANALYSIS
653 $aMONOSACCHARIDE ANALYIS
653 $aQUILLAJA BRASILIENSIS
700 1 $aBENNADJI, Z.
700 1 $aFERREIRA, F.
700 1 $aOLIVARO, C.
773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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Registro original : |
INIA Tacuarembó (TBO) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
29/10/2020 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
MONDINO, P.; ALTIER, N.; VERO, S.; PEREYRA, S.; FOLCH, C. |
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Afiliación : |
PEDRO MONDINO, Cátedra de Fitopatología, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República (UdelaR), Montevideo.; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA VERO, Cátedra de Microbiología, Departamento de Biociencias, Facultad de Química. Universidad de la República (UdelaR), Uruguay.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLAUDIA FOCH, Técnico en empresa Lage y Cia SA. Camino Carrasco 6348. Montevideo. |
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Título : |
Control biológico en el Uruguay. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
In: Bettiol, W.; Rivera, M. Mondino, P.; Montealegre, J.; Colmenares, Y. Eds. 2014 Control biológico de enfermedades de plantas en América Latina, UdelaR, Facultad de Agronomía, Montevideo, Uruguay. |
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Páginas : |
p. 339-368. |
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ISBN : |
978-9974-0-1091-8 |
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Idioma : |
Español |
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Thesagro : |
CONTROL BIOLÓGICO; CONTROL DE PLAGAS; URUGUAY. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14783/1/Control-Biologico-de-enfermedades-de-plantas-en-Uruguy.cap.17.2014.pdf
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Marc : |
LEADER 00723naa a2200217 a 4500
001 1042268
005 2020-10-29
008 2014 bl uuuu u00u1 u #d
020 $a978-9974-0-1091-8
100 1 $aMONDINO, P.
245 $aControl biológico en el Uruguay.$h[electronic resource]
260 $c2014
300 $ap. 339-368.
650 $aCONTROL BIOLÓGICO
650 $aCONTROL DE PLAGAS
650 $aURUGUAY
700 1 $aALTIER, N.
700 1 $aVERO, S.
700 1 $aPEREYRA, S.
700 1 $aFOLCH, C.
773 $tIn: Bettiol, W.; Rivera, M. Mondino, P.; Montealegre, J.; Colmenares, Y. Eds. 2014 Control biológico de enfermedades de plantas en América Latina, UdelaR, Facultad de Agronomía, Montevideo, Uruguay.
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INIA La Estanzuela (LE) |
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